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phr pefegfp vector47  (Addgene inc)


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    Structured Review

    Addgene inc phr pefegfp vector47
    Phr Pefegfp Vector47, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phr+pefegfp+vector47/pHR-pEF-EGFP+(Plasmid+%2367952)/pm41832176-298-4-6
    Average 93 stars, based on 2 article reviews
    phr pefegfp vector47 - by Bioz Stars, 2026-11
    93/100 stars

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    Plasmid Preparation:

    Article Title: Cell type-specific enhancers regulate IL-22 expression in innate and adaptive type 3 lymphoid cells.
    Article Snippet: For Lenti-TRE3G-dCas9-KRAB-P2A-mCherry construct, tetracycline inducible TRE3G promoter was amplified from c3GIC946 (Addgene Plasmid #62191) vector adding EcoR1 restriction site at 5’ end and Mlu1 followed by BamH1 restriction enzyme sites at the 3’ end. .. Next, TRE3G promoter and pHR-pEFEGFP vector47 (Addgene Plasmid #67952) were digested with EcoR1+BamH1 and ligated, resulting in pHR-TRE3G-EGFP vector. .. Lastly, dCas9-KRAB-P2A-mcherry cassette was swapped from pHR-SFFVKRAB-dCas9-P2A-mCherry48 (Addgene Plasmid #60954) into pHR-TRE3G-EGFP vector from above using Mlu1 and Not1 restriction sites. sgRNAs targeting Il22 promoter, E22-1, E22-2, twin-E22-2, or scrambled sgRNA were cloned downstream of the U6 promoter in sgOpti49 (Addgene Plasmid #85681).



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    93
    Addgene inc phr pefegfp vector47
    Phr Pefegfp Vector47, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phr+pefegfp+vector47/pHR-pEF-EGFP+(Plasmid+%2367952)/pm41832176-298-4-6
    Average 93 stars, based on 1 article reviews
    phr pefegfp vector47 - by Bioz Stars, 2026-11
    93/100 stars
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